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URL:
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http://biocore.unl.edu/primer/primerPI.html
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What you can do:
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Design PCR primers for protein-protein interaction experiments.
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Highlights:
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- The existing PCR primer design tools cannot be directly used for the design of primers in protein interaction experiments, since proteins may have transmembrane domains (TMDs) and/or a signal peptide that must be excluded from experiments.
- Furthermore, it is frequently the case that a short restriction sequences must be added to each primer in order to clone PCR products into a given destination vectors for expression.
- DePIE is a web-based primer design tool developed to address these deficiencies.
- It was initially developed to design primers for the yeast two-hybrid assays, but can be applied into any other assays as long as the primers are needed.
- The program takes as input NCBI protein accession numbers and returns primer information including nucleotide sequences, thermodynamic melting temperature of the nucleotide sequences and the target positions.
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Keywords:
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- PCR primers
- PCR primer design tool
- Polymerase Chain Reaction primers design tool
- yeast Two-Hybrid
- protein-protein interactions
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Literature and Tutorials:
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PubMed Link: DePIE -- Designing Primers for Protein Interaction Experiments
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