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URL:
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http://www.mdl.dk/publicationsnormfinder.htm
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What you can do:
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Identify the optimal normalization gene among a set of candidates.
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Highlights:
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- “NormFinder” is an algorithm for identifying the optimal normalization gene among a set of candidates.
- It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design.
- NormFinder can analyze expression data obtained through any quantitative method e.g. real time RT-PCR and microarray based expression analysis.
- ”NormFinder.xla” is an Add-in for Microsoft Excel which adds the NormFinder functionality directly to the Excel software package for easy use.
- “NormFinder” provides a stability value for each gene, which is a direct measure for the estimated expression variation enabling the user to evaluate the systematic error introduced when using the gene for normalization.
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Keywords:
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- real-time reverse transcription PCR
- RT-PCR
- qRT-PCR
- gene expression data normalization tool
- RT-PCR expression profiling
- house-keeping genes
- microarray data analysis tool
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Literature and Tutorials:
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Link to the Manual: NormFinder -- tool for identifying normalization genes
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