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URL:
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http://publish.yorku.ca/~pjohnson/AssemblyPCRoligomaker.html
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What you can do:
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Design of oligodeoxynucleotides for the PCR based construction of long DNA molecules.
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Highlights:
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- This program has been specifically designed to aid in the construction of DNA molecules that are to be used for the production of RNA molecules by in vitro synthesis with T7 RNA polymerase.
- The input for Assembly PCR Oligo Maker is either the desired DNA sequence to be made or an RNA sequence.
- If RNA is the input, the program first determines the DNA sequence necessary to produce the desired RNA molecule.
- The program then determines the sequences of all the oligodeoxynucleotides necessary for a two-step assembly PCR-based synthesis of the desired DNA molecule.
- The oligodeoxynucleotide sequences outputted are designed to have a uniform melt temperature and are checked for regions of overlap outside of the desired priming regions necessary for the PCR reaction.
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Keywords:
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- PCR
- Polymerase Chain Reaction
- PCR primer designing tool
- DNA primers
- PCR oligo designing tool
- Oligodeoxyribonucleotides
- RNA biosynthesis
- assembly PCR
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Literature and Tutorials:
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PubMed Link: Assembly PCR oligo maker -- a tool for designing oligodeoxynucleotides for constructing long DNA molecules for RNA production
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