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URL:
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http://genome.ewha.ac.kr/ASePCR/
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What you can do:
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Carry out simulated RT-PCR to detect transcript variants.
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Highlights:
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- ASePCR is an efficient web-based application that emulates RT-PCR in various tissues.
- It estimates the amplicon size for a given primer pair based on the transcript models identified by the reverse e-PCR program of the NCBI.
- The tissue specificity of each PCR band is deduced from the tissue information of expressed sequence tag (EST) sequences compatible with each transcript structure.
- The output page shows PCR bands like a gel electrophoresis in various tissues. Each band in the output picture represents a putative isoform that could happen in a tissue-specific manner.
- It also shows the EST alignment and tissue information in the genome browser.
- Furthermore, the user can compare the AS patterns of orthologous genes in other species.
- It will be a valuable web resource to explore the transcriptome diversity associated with different tissues and organs in multiple species.
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Keywords:
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- RT-PCR
- e-PCR
- Expressed Sequence Tags
- tissue-specific gene expression
- transcript variants
- alternative splicing
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Literature and Tutorials:
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PubMed Link: ASePCR -- alternative splicing electronic RT–PCR in multiple tissues and organs
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