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URL:
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http://129.43.22.27/~primer/
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What you can do:
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Design PCR primers for large-scale resequencing of exons.
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Highlights:
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- Using the Primer3 algorithm, EasyExonPrimer is a web-based software that automates the design of PCR primers to amplify exon sequences from genomic DNA.
- It masks repeats and known single nucleotide polymorphism (SNP) sites in the genome and designs standardized primers using optimized conditions.
- Users can input genes by RefSeq mRNA ID, gene name or keyword.
- The primer design is optimized for large-scale resequencing of exons.
- For exons larger than 1 kb, the user has the option of breaking the exon sequence down into overlapping smaller fragments.
- All primer pairs are then verified using the In-Silico PCR software to test for uniqueness in the genome.
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Keywords:
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- PCR primers design tool
- exon amplification primers design tool
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Literature and Tutorials:
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PubMed Link: EasyExonPrimer -- automated primer design for exon sequences
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