|
URL:
|
http://www.cstl.nist.gov/div831/strbase//AutoDimerHomepage/AutoDimerProgramHomepage.htm
|
|
What you can do:
|
Examine multiple PCR primers for primer-dimer and hairpin structures.
|
|
Highlights:
|
- AutoDimer software was developed to rapidly screen previously selected PCR primers for primer-dimer and hairpin interactions in short DNA oligomers (< 30 nucleotides).
- After the screening is completed, a score is assigned to potential duplex interactions exceeding a user-defined threshold.
- Additional criteria of predicted melting temperature (Tm) and free energy of melting (deltaG) are included for further ranking.
- Sodium counterion and total stand concentrations can be adjusted for the Tm and deltaG calculations.
|
|
Keywords:
|
- multiplex PCR primers evaluation tool
- PCR primer quality
- primer-dimer prediction tool
- primer hairpin structure prediction tool
|
|
Literature and Tutorials:
|
PubMed Link: AutoDimer -- a screening tool for primer-dimer and hairpin structures
|
