|
URL:
|
http://scprimer.cpmc.columbia.edu/SCPrimerApp.cgi
|
|
What you can do:
|
Design degenerate PCR primers for large groups of related DNA sequences.
|
|
Highlights:
|
- The web application allows design of degenerate primers from multiple alignments nucleic acid sequences in fasta or ClustalW format.
- The program first computes phylogenetic trees to identify candidate primers, and then uses a greedy SCP solving algorithm to identify the minimum set that will amplify all members of the alignment.
- SCPrimer was created to address the specific needs of virologists and bacteriologists in creating primers for pathogen detection.
- The web application requires a simple registration, after which the user will be able to store their results and monitor progress of design jobs.
|
|
Keywords:
|
- degenerate PCR primer design tool
- multiplex PCR primers design tool
- Polymerase chain reaction
|
|
Literature and Tutorials:
|
PubMed Link: Greene SCPrimer -- a rapid comprehensive tool for designing degenerate primers from multiple sequence alignments
|
