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URL:
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http://www.aad.gov.au/default.asp?casid=11381
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What you can do:
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Design PCR primers on aligned groups of DNA sequences.
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Highlights:
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- The most important application for Amplicon is the design of 'group-specific' PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups.
- As well as dealing with group-specific PCR, Amplicon is useful for developing species-specific, allele-specific or any ‘normal’ PCR primer sets.
- Amplicon works with sequences including degenerate sites and gaps.
- Amplicon is freely available as a script that will run on any platform with Python 2.3 installed.
- It is also available as a Windows executable that can be downloaded freely.
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Keywords:
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- PCR primers
- PCR primer design tool
- Polymerase Chain Reaction primers design tool
- group specific PCR primers
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Literature and Tutorials:
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PubMed Link: Amplicon --software for designing PCR primers on aligned DNA sequences
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