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URL:
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http://oblab.cs.nchu.edu.tw:8080/WebSDL/
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What you can do:
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Use this web-based application system to apply the sequential deletion method to locate the functional domain of a protein and generate groups of primers.
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Highlights:
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- The sequential deletion method is generally used to locate the functional domain of a protein.
- With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments.
- This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants.
- This saves much time and money for those who use the sequential deletion method in their research.
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Keywords:
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- Automation
- DNA Primers
- DNA, Complementary/*metabolism
- Gene Deletion
- Mutagenesis
- Mutation
- Polymerase Chain Reaction/*methods
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA
- User-Computer Interface
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Literature and Tutorials:
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PubMed Link: NTMG (N-terminal Truncated Mutants Generator for cDNA) -- An automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
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