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URL:
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http://consurf.tau.ac.il/
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What you can do:
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Automatically calculate evolutionary conservation scores of key amino acid residues and map them on protein structures.
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Highlights:
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- ConSurf-DB is a repository for evolutionary conservation analysis of the proteins of known structures in the Protein Data Bank (PDB).
- Sequence homologues of each of the PDB entries were collected and aligned using standard methods.
- The evolutionary conservation of each amino acid position in the alignment was calculated using the Rate4Site algorithm, implemented in the ConSurf web server.
- The algorithm takes into account the phylogenetic relations between the aligned proteins and the stochastic nature of the evolutionary process explicitly.
- Rate4Site assigns a conservation level for each position in the multiple sequence alignment using an empirical Bayesian inference.
- Visual inspection of the conservation patterns on the 3D structure often enables the identification of key residues that comprise the functionally important regions of the protein.
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Keywords:
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- protein sequence analysis tool
- protein conserved sequences
- protein functional sites
- protein binding sites
- protein catalytic sites
- protein functional site mapping tool
- protein evolution
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Literature and Tutorials:
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PubMed Link: ConSurf 2005 -- the projection of evolutionary conservation scores of residues on protein structures
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2009 Update: The ConSurf-DB: pre-calculated evolutionary conservation profiles of protein structures
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