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URL:
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http://alpha10.bioch.virginia.edu/crp/
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What you can do:
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Design and interpret experiments to identify protein phosphorylation sites by Edman sequencing of unseparated peptides.
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Highlights:
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- The CRP (Cleavage of Radiolabeled Phosphoproteins) program guides the design and interpretation of experiments to identify protein phosphorylation sites by Edman sequencing of unseparated peptides.
- CRP analysis of a phosphoprotein's sequence accelerates this process by omitting the separation step: given a protein sequence of interest, the CRP program performs an in silico proteolytic cleavage of the sequence and reports the predicted Edman cycles in which radioactivity would be observed if a given serine, threonine or tyrosine were phosphorylated.
- Experimentally observed cycles containing 32P can be compared with CRP predictions to confirm candidate sites and/or explore the ability of additional cleavage experiments to resolve remaining ambiguities.
- To reduce ambiguity, the phosphorylated residue (P-Tyr, P-Ser or P-Thr) can be determined experimentally, and CRP will ignore sites with alternative residues.
- CRP also provides simple predictions of likely phosphorylation sites using known kinase recognition motifs.
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Keywords:
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- phosphorylation sites
- phosphorylation site prediction tool
- protein phosphorylation
- kinase recognition motifs
- Phosphoproteins
- Phosphoserine analysis
- Phosphothreonine analysis
- Phosphotyrosine analysis
- protein modification sites
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Literature and Tutorials:
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PubMed Link: CRP -- Cleavage of Radiolabeled Phosphoproteins
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