E-RNAi -- a web application to design optimized RNAi constructs
What you can do:
Evaluate and design optimized dsRNA constructs.
Highlights:
- E-RNAi provides a computational resource for the optimized design and evaluation of RNAi reagents.
- The 2010 update now covers 12 genomes, including Drosophila, Caenorhabditis elegans, human, emerging model organisms such as Schmidtea mediterranea and Acyrthosiphon pisum, as well as the medically relevant vectors Anopheles gambiae and Aedes aegypti.
- The web service calculates RNAi reagents based on the input of target sequences, sequence identifiers or by visual selection of target regions through a genome browser interface.
- It identifies optimized RNAi target-sites by ranking sequences according to their predicted specificity, efficiency and complexity.
- E-RNAi also facilitates the design of secondary RNAi reagents for validation experiments, evaluation of pooled siRNA reagents and batch design.
- Results are presented online, as a downloadable HTML report and as tab-delimited files.
Keywords:
- RNA interference
- RNAi
- small interfering RNA
- double-stranded RNA
- dsRNA
- dsRNA design tool
- RNAi probe design tool
- RNAi probes
- siRNA design tool
Literature & Tutorials:
This record last updated: 06-04-2010