MaXIC-Q -- Automated quantitation tool for stable isotope labeling techniques
What you can do:
Analyze differential protein expression in large data sets.
Highlights:
- MaXIC-Q Web is a web server for quantitation analysis of large-scale datasets generated from proteomics experiments using various stable isotope-labeling techniques, e.g. SILAC, ICAT and user-developed labeling methods.
- It accepts spectral files in the standard mzXML format and search results from SEQUEST, Mascot and ProteinProphet as input.
- It uses statistical and computational methods to construct two kinds of elution profiles for each ion, namely, PIMS (projected ion mass spectrum) and XIC (extracted ion chromatogram) from MS data.
- Toward accurate quantitation, a stringent validation procedure is performed on PIMSs to filter out peptide ions interfered with co-eluting peptides or noise.
- The areas of XICs determine ion abundances, which are used to calculate peptide and protein ratios.
- Since MaXIC-Q Web adopts stringent validation on spectral data, it achieves high accuracy so that manual validation effort can be substantially reduced.
- It provides various visualization diagrams and comprehensive quantitation reports so that users can conveniently inspect quantitation results.
Keywords:
- protein quantitation
- stable isotope labeling
- LC-MS
- liquid chromatography-mass spectrometry
- projected ion mass spectrum
- PIMS
- extracted ion chromatogram
- XIC
This record last updated: 04-21-2014