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DNA Analysis Tools
This workshop will cover major bioinformatics tools for nucleotide analysis that are freely available on the Internet:
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PCR primer construction & analysis
- NetPrimer: a tool for analyzing primers
- Primer3Plus: a tool for creating primers
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Restriction digestion & mapping
- NEBcutter: a DNA sequence tool to find large, non-overlapping, open reading frames and sites for all restriction enzymes
- Webcutter: software for restriction mapping nucleotide sequences
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Sequence formatting & manipulation
- READSEQ: converts input DNA/AA sequence to specified format
- Sequence Manipulation Suite: a collection of JavaScript programs for generating, formatting, and analyzing short DNA and protein sequences
Participants will learn how to answer questions like:
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How do I create PCR primers for my sequence of interest?
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How can I check that the primers I already created are actually the best to use?
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How do I locate a specific DNA pattern in my sequence?
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How many open reading frames (ORFs) are present in my sequence?
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Which restriction enzymes will excise the largest ORF in my sequence?
This class is approved for AMA Category 2 CME credit.
If you have questions, contact the HSLS Molecular Biology Information Service:
Class PowerPoint Presentation
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Wednesday, December 02
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1 p.m. - 3 p.m.
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(Falk Library, Conference Room B)
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Instructor: Carrie Iwema, PhD, MLS
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