Partek Flow

HSLS Live Classes

Single Cell RNA-Seq Data Analysis using Partek Flow

Level: Novice

This workshop is on single cell RNASeq analysis with multiple samples using HSLS-licensed Partek Flow software.

Target Audience: 

Experimental biologists seeking to learn how to analyze the single cell data generated through experiments or retrieved from a publication or a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither computer programming experience nor familiarity with the command-line interface is required.

Upon completing this class, you should be able to:
  • analyze from Raw data (FASTQ files)
  • import FASTQ files and run Cellranger software to generate single cell count matrix
  • analyze from Count Matrix data
  • import single cell count matrix
  • perform quality checks (QC) on single-cell data
  • filter and normalize the data
  • generate PCA and t-SNE, UMAP plots to visualize data
  • identify cell populations, and biomarkers in the multisample data
  • compute differential gene expressions between two cell populations and/or samples

Event Details

Date:
Time: to
Mode: Zoom
Location: Online, Online - synchronous
Instructor: Ansuman Chattopadhyay
Class materials will be shared with attendees.
This class will be recorded and shared with attendees.
What should you do before attending this class:

Register for Partek Flow software.


Register for this class