This workshop teaches bulk RNA-Seq data analysis using CLC Genomics Workbench software. Upon registration, you will receive links to workshop materials that you can view on your schedule.
Target Audience
Experimental biologists seeking to analyze bulk RNA-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user interface, so neither programming experience nor familiarity with the command-line interface is required.
Upon completing this class, you should be able to:
- access the CLCbio Genomics Server hosted by Pitt CRC
- import RNA-Seq FASTQ reads from a GEO dataset
- assess the quality of RNA-Seq data
- align reads to a reference genome
- estimate known gene and transcript expression
- perform differential expression analysis
- visualize data by generating PCA and heatmaps