ChIP-Seq & Partek Flow

Workshop Objective:

This 3-hour hands-on workshop provides a brief introduction to techniques, platforms, and methods used in chromatin profiling experiments, including Transcription Factor / Histone ChIP-Seq and ATAC-Seq. We will focus on data analysis using HSLS-licensed Partek Flow software.

Participants will learn how to

  • import raw data from a GEO dataset
  • align ChIP-Seq reads to a reference genome
  • identify and annotate peaks
  • visualize and summarize the results
  • search for known transcription factor binding motifs
  • integrate ChIP-Seq results with bulk RNA-Seq data

Target Audience:

Experimental biologists seeking to analyze ChIP-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither programming experience nor familiarity with command line interface is required.

Workshop Requirements:

Workshop Guide:

Suggested Reading:

Attribution:

Please include the following statement in the acknowledgments section for all publications, posters, and presentations: Data analysis was performed using {name of software} software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh.

Class Type

Upcoming Classes

ChIP-Seq & Partek Flow

Event Details

Instructor: Ansuman Chattopadhyay, Srilakshmi Chaparala
Date:
Time: to
Location: Scaife Hall, Falk Library, Classroom 2
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