Workshop Objective:
This 3-hour hands-on workshop provides a brief introduction to techniques, platforms, and methods used in chromatin profiling experiments, including Transcription Factor / Histone ChIP-Seq and ATAC-Seq. We will focus on data analysis using HSLS-licensed Partek Flow software.
Participants will learn how to
- import raw data from a GEO dataset
- align ChIP-Seq reads to a reference genome
- identify and annotate peaks
- visualize and summarize the results
- search for known transcription factor binding motifs
- integrate ChIP-Seq results with bulk RNA-Seq data
Target Audience:
Experimental biologists seeking to analyze ChIP-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither programming experience nor familiarity with command line interface is required.
Workshop Requirements:
Workshop Guide:
Suggested Reading:
Attribution:
Please include the following statement in the acknowledgments section for all publications, posters, and presentations: Data analysis was performed using {name of software} software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh.