Workshop Objective:
This is a 4-hour workshop. The morning session (10am - 12pm) provides a brief introduction to techniques, platforms, and methods used in chromatin profiling experiments, including Transcription Factor / Histone ChIP-Seq and ATAC-Seq. The afternoon session (1pm - 3pm) focuses on hands-on data analysis practice using HSLS-licensed CLC Genomics Workbench, Partek Flow, and TRANSFAC/Match software.
Participants will learn how to
- import NGS reads from a GEO dataset
- align ChIP-Seq reads to a reference genome
- identify and annotate peaks
- visualize and summarize the ChIP-Seq results
- search for known transcription factor binding motifs using TRANSFAC/Match software
Target Audience:
Experimental biologists seeking to analyze ChIP-Seq data generated through experiments or retrieved from a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither programming experience nor familiarity with command line interface is required.
Workshop Requirements:
Workshop Guide:
Suggested Reading:
Attribution:
Please include the following statement in the acknowledgments section for all publications, posters, and presentations: Data analysis was performed using {name of software} software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh.