Gene Regulation

Workshop Objective:

This 3-hr hands-on workshop provides an overview of resources and search strategies on transcriptional regulation. Emphasis will be given to HSLS-licensed TRANSFAC/Match and Correlation Engine software. Participants will learn to

  • retrieve promoter sequence for a gene of interest using UCSC Genome browser
  • locate transcription factor binding sites present in a query sequence using Match
  • visualize the regulatory regions present in the human genome generated by the Encyclopedia of DNA Elements (ENCODE) project
  • browse through epigenetic biochemical markers
  • start with a GEO data set and find correlated studies using Correlation Engine 

Target Audience:

Experimental biologists studying epigenetic regulation on gene expression and interested in analyzing promoter and enhancer sequences. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither programming experience nor familiarity with command line interface is required.

Workshop Requirements:

Workshop Guide:

Attribution:

Please include the following statement in the acknowledgments section for all publications, posters, and presentations: Data analysis was performed using {name of software} software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh.

Class Type

Upcoming Classes

Gene Regulation

Event Details

Instructor: Ansuman Chattopadhyay, Srilakshmi Chaparala
Date:
Time: to
Location: Scaife Hall, Falk Library, Classroom 2
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