In this workshop, we will cover the advanced single-cell RNASeq analysis techniques such as CITE Seq and Spatial Transcriptomics.
Participants will learn how to
- import 10x Genomics CITE-Seq and Spatial Transcriptomics (Visium) data to PartekFlow
- merge expression data with histological information
- filter and normalize the data
- generate PCA, t-SNE, and UMAP plots to visualize data
- identify cell populations, biomarkers, and compute differential gene expressions between two cell populations