Primer3 -- An online tool for PCR primer design

What you can do:
Design optimum PCR primers from nucleotide sequences.
Highlights:
  • Select optimal primer pairs for PCR reactions using user-specifiable parameters such as %GC content, melting temperature (Tm), and many more constraints.
  • Determine primer-dimer possibilities.
  • Select "internal oligo" intended to be used as hybridization probe to detect PCR product after amplification.
  • Uses DNA sequence in FASTA format.
Keywords:
  • Polymerase Chain Reaction
  • PCR
  • DNA amplification
  • Gene amplification
  • PCR primers
  • PCR Primer Design tool
This record last updated: 05-06-2005
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