siRNA Selection Server -- an automated siRNA oligonucleotide prediction server
What you can do:
Identify potential siRNA target sites within an mRNA sequence.
Highlights:
- This tool incorporates published design rules and presents the scientist with information about uniqueness of the 21mers within the genome, thermodynamic stability of the double stranded RNA duplex, GC content, presence of SNPs and other features that may contribute to the effectiveness of a siRNA.
- After entering your sequence, choosing a pattern for your oligo nucleotides, and selecting various filter methods, you will be presented with a list of oligos matching your pattern. From this list, select those oligos you would like to consider further, based on their position within your sequence, the SNPs, and other criteria.
- Your results will be available directly on the web and/or will be emailed to you when they are ready.
- Registration (free) is required for using the tool.
Keywords:
- Small Interfering RNAs
- siRNA
- siRNA design tools
- RNA Interference
- knock down expression
- RNAi
- gene silencing
Literature & Tutorials:
PubMed Link: siRNA Selection Server
This record last updated: 05-06-2005