SNPbox -- Web-based High-throughput Primer Design from Gene to Genome

URL:

http://www.snpbox.org/

What you can do:

Automatically design PCR primers for large-scale amplification and sequencing projects aimed at constructing single nucleotide polymorphisms (SNPs) maps.

Highlights:

Based on Primer3 algorithm, SNPbox can be used for genomic sequences up to 200 kb.
The server also hosts the data of SNPbox analysis of all Ensembl exons.
The data of this genome-wide SNPbox application can be visualized in Ensembl's ContigView through a DAS (Distributed Annotation System) annotation server.
For its 2005 release, SNPbox automates and standardizes the generation of PCR primers and is used in the strategy for constructing single nucleotide polymorphisms (SNPs) maps.
Primer conditions were chosen such that PCR amplifications are uniform for each PCR amplicon facilitating the use of high-throughput genetic platforms.
SNPbox can also be used for the design of primer sets for mutation analysis, STR marker genotyping and microarray oligo design.

Keywords:

PCR primers
PCR primer design tool
SNP genotyping primer design tool
mutation analysis primers
microarray oligo design tool
Short Tandem Repeat markers (STR) design tool

Literature & Tutorials:

PubMed Link to the original article (2004): SNPbox -- Web-based High-throughput Primer Design from Gene to Genome
PubMed Link to 2005 update: SNPbox: a modular software package for large-scale primer design

This record last updated: 08-14-2006

Report a missing or misdirected URL.