Identify and extract nucleotide sequence around the start of genes (promoter region) and their translation initiation site (TIS).
Highlights:
Using information provided by the National Center for Biotechnology Information's (NCBI's) LocusLink, FIE2 identifies the 5'-most end of a gene on its respective chromosome based on alignment of a selected set of mRNAs representative of the gene.
FIE2 then uses available human genome sequence information to extract the desired sequences.
The accuracy of the information extracted is therefore limited by the accuracy and completeness of the sequence annotation and sequence alignment provided by LocusLink.
In addition, multiple TIS positions are also occasionally presented.