DePIE -- Designing Primers for Protein Interaction Experiments
What you can do:
Design PCR primers for protein-protein interaction experiments.
Highlights:
- The existing PCR primer design tools cannot be directly used for the design of primers in protein interaction experiments, since proteins may have transmembrane domains (TMDs) and/or a signal peptide that must be excluded from experiments.
- Furthermore, it is frequently the case that a short restriction sequences must be added to each primer in order to clone PCR products into a given destination vectors for expression.
- DePIE is a web-based primer design tool developed to address these deficiencies.
- It was initially developed to design primers for the yeast two-hybrid assays, but can be applied into any other assays as long as the primers are needed.
- The program takes as input NCBI protein accession numbers and returns primer information including nucleotide sequences, thermodynamic melting temperature of the nucleotide sequences and the target positions.
Keywords:
- PCR primers
- PCR primer design tool
- Polymerase Chain Reaction primers design tool
- yeast Two-Hybrid
- protein-protein interactions
Literature & Tutorials:
This record last updated: 06-16-2005