NormFinder -- tool for identifying normalization genes

URL:
http://moma.dk/normfinder-software
What you can do:
Identify the optimal normalization gene among a set of candidates.
Highlights:
  • x93NormFinderx94 is an algorithm for identifying the optimal normalization gene among a set of candidates.
  • It ranks the set of candidate normalization genes according to their expression stability in a given sample set and given experimental design.
  • NormFinder can analyze expression data obtained through any quantitative method e.g. real time RT-PCR and microarray based expression analysis.
  • x94NormFinder.xlax94 is an Add-in for Microsoft Excel which adds the NormFinder functionality directly to the Excel software package for easy use.
  • x93NormFinderx94 provides a stability value for each gene, which is a direct measure for the estimated expression variation enabling the user to evaluate the systematic error introduced when using the gene for normalization.
Keywords:
  • real-time reverse transcription PCR
  • RT-PCR
  • qRT-PCR
  • gene expression data normalization tool
  • RT-PCR expression profiling
  • house-keeping genes
  • microarray data analysis tool
Literature & Tutorials:
Link to the article in PMC: Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets
This record last updated: 08-05-2020
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