dsCheck -- highly sensitive off-target search software for double-stranded RNA-mediated RNA interference
What you can do:
Estimate off-target effects by the long double-stranded RNA used in RNAi studies.
Highlights:
- The software simulates the dsRNA cleaving process and investigates individual 19 nt substrings of the long dsRNA.
- Subsequently, the software promptly enumerates a list of potential off-target gene candidates based on the order of off-target effects using its novel algorithm, which significantly improves both the efficiency and the sensitivity of the homology search.
- The website not only provides a rigorous off-target search to verify previously designed dsRNA sequences but also presents x91off-target minimizedx92 dsRNA design, which is essential for reliable experiments in RNAi-based functional genomics.
Keywords:
- RNA interference
- RNAi
- small interfering RNA
- double-stranded RNA
- dsRNA
- dsRNA design tool
- dsRNA verification tool
- RNAi probe design tool
- RNAi probes
- siRNA design tool
Literature & Tutorials:
This record last updated: 12-20-2005