ASePCR -- alternative splicing electronic RTx96PCR in multiple tissues and organs
What you can do:
Carry out simulated RT-PCR to detect transcript variants.
Highlights:
- ASePCR is an efficient web-based application that emulates RT-PCR in various tissues.
- It estimates the amplicon size for a given primer pair based on the transcript models identified by the reverse e-PCR program of the NCBI.
- The tissue specificity of each PCR band is deduced from the tissue information of expressed sequence tag (EST) sequences compatible with each transcript structure.
- The output page shows PCR bands like a gel electrophoresis in various tissues. Each band in the output picture represents a putative isoform that could happen in a tissue-specific manner.
- It also shows the EST alignment and tissue information in the genome browser.
- Furthermore, the user can compare the AS patterns of orthologous genes in other species.
- It will be a valuable web resource to explore the transcriptome diversity associated with different tissues and organs in multiple species.
Keywords:
- RT-PCR
- e-PCR
- Expressed Sequence Tags
- tissue-specific gene expression
- transcript variants
- alternative splicing
Literature & Tutorials:
This record last updated: 12-22-2005