miRNAMap 2.0 -- genomic maps of microRNAs in metazoan genomes
What you can do:
Search for information on the published and predicted miRNA in vertebrate genomes.
Highlights:
- MicroRNAs (miRNAs) are small non-coding RNA molecules that can negatively regulate gene expression and thus control numerous cellular mechanisms.
- This work develops a resource, miRNAMap 2.0, for collecting experimentally verified microRNAs and experimentally verified miRNA target genes in human, mouse, rat and other metazoan genomes.
- Three computational tools, miRanda, RNAhybrid and TargetScan, were employed to identify miRNA targets in 3'-UTR of genes as well as the known miRNA targets.
- Various criteria for filtering the putative miRNA targets are applied to reduce the false positive prediction rate of miRNA target sites.
- Additionally, miRNA expression profiles can provide valuable clues on the characteristics of miRNAs, including tissue specificity and differential expression in cancer/normal cell.
- Therefore, quantitative polymerase chain reaction experiments were performed to monitor the expression profiles of 224 human miRNAs in 18 major normal tissues in human.
- The negative correlation between the miRNA expression profile and the expression profiles of its target genes typically helps to elucidate the regulatory functions of the miRNA.
- The interface is also redesigned and enhanced.
Keywords:
- microRNAs
- microRNA genes
- miRNAs
- miRNA targets
- miRNA target prediction tool
- miRNA maps
- mammalian miRNA
- vertebrate miRNA
- non-coding RNA prediction tool
- miRNA expression profiles
- RNA interferences
- RNAi
- non-coding RNA
Literature & Tutorials:
This record last updated: 03-06-2008