Oryza Tag Line -- a phenotypic mutant database for the Genoplante rice insertion line library
What you can do:
Find info from the phenotypic characterization of a library of 30,000 T-DNA enhancer trap (ET) insertion lines of rice (Oryza sativa L cv. Nipponbare)
Highlights:
- OTL structure facilitates forward genetic search for specific phenotypes, putatively resulting from gene disruption, and/or for GUSA or GFP reporter gene expression patterns, reflecting ET-mediated endogenous gene detection.
- In the latest version, OTL gathers the detailed morpho-physiological alterations observed during field evaluation and specific screens in a first set of 13,928 lines.
- Detection of GUS or GFP activity in specific organ/tissues in a subset of the library is also provided.
- Search in OTL can be achieved through trait ontology category, organ and/or developmental stage, keywords, expression of reporter gene in specific organ/tissue as well as line identification number.
- OTL now contains the description of 9721 mutant phenotypic traits observed in 2636 lines and 1234 GUS or GFP expression patterns.
- Each insertion line is documented through a generic passport data including production records, seed stocks and FST information.
- 8004 and 6101 of the 13,928 lines are characterized by at least one T-DNA and one Tos17 FST, respectively that OTL links to the rice genome browser OryGenesDB.
Keywords:
- rice T-DNA insertion mutants
- rice insertional mutagenesis
- rice mutants
- Oryza sativa mutants
- rice insertion mutants
- rice Flanking Sequence Tags
- rice phenotypes
- rice GUS/GFP expression assays
Literature & Tutorials:
This record last updated: 04-07-2008