PrimerStation -- a highly specific multiplex genomic PCR primer design server for the human genome
What you can do:
Design highly specific and accurate multiplex genomic PCR primer for human genome.
Highlights:
- PrimerStation is a web service that calculates primer sets guaranteeing high specificity against the entire human genome.
- High accuracy is achieved by using the hybridization ratio of primers in liquid solution.
- PrimerStation can select highly specific primers suitable for multiplex PCR by seeking a wider temperature range that minimizes the possibility of cross-reaction.
- It also allows users to add heuristic rules to the primer design, e.g. the exclusion of single nucleotide polymorphisms (SNPs) in primers, the avoidance of poly(A) and CA-repeats in the PCR products, and the elimination of defective primers using the secondary structure prediction.
Keywords:
- PCR primers design tool
- multiplex genomic PCR primer design tool
- human genome amplification
Literature & Tutorials:
This record last updated: 08-05-2006