Primaclade -- a flexible tool to find conserved PCR primers across multiple species
What you can do:
Find conserved PCR primers across multiple species based on a multiple nucleotide alignments.
Highlights:
- Primaclade is a web-based application that accepts a multiple species nucleotide alignment file as input and identifies a set of polymerase chain reaction (PCR) primers that will bind across the alignment.
- It iteratively runs the Primer3 application for each alignment sequence and collates the results.
- Primaclade creates an HTML results page that recaps the original alignment, provides a consensus sequence and lists primers for each alignment area, with primers color-coded to reflect the level of degeneracy in the primer.
- The user may adjust a number of parameters to improve and refine the primers.
Keywords:
- PCR primers design tool
- degenerate PCR primers
- conserved sequence amplification
Literature & Tutorials:
This record last updated: 08-07-2006