AutoDimer -- a screening tool for primer-dimer and hairpin structures
What you can do:
Examine multiple PCR primers for primer-dimer and hairpin structures.
Highlights:
- AutoDimer software was developed to rapidly screen previously selected PCR primers for primer-dimer and hairpin interactions in short DNA oligomers (< 30 nucleotides).
- After the screening is completed, a score is assigned to potential duplex interactions exceeding a user-defined threshold.
- Additional criteria of predicted melting temperature (Tm) and free energy of melting (deltaG) are included for further ranking.
- Sodium counterion and total stand concentrations can be adjusted for the Tm and deltaG calculations.
Keywords:
- multiplex PCR primers evaluation tool
- PCR primer quality
- primer-dimer prediction tool
- primer hairpin structure prediction tool
Literature & Tutorials:
This record last updated: 08-07-2006