PerlPrimer -- cross-platform, graphical primer design for standard, bisulphite and real-time PCR
What you can do:
Design various PCR primers on PC, Mac or Linux based system.
Highlights:
- PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing.
- It aims to automate and simplify the process of primer design.
- PerlPrimer's current features include the following:
- Calculation of possible primer-dimers
- Retrieval of genomic or cDNA sequences from Ensembl (including both sequences automatically for QPCR)
- Ability to BLAST search primers using the NCBI server or a local server
- Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications.
- ORF and CpG island detection algorithms
- Ability to add cloning sequences to primers, automatically adjusted to be in-frame
- QPCR primer design without manual intron-exon boundary entry
Keywords:
- PCR primers
- PCR primer design tool
- Polymerase Chain Reaction primers design tool
- bisulphite primers
- Methylation PCR Primers
- real-time PCR primers
- QPCR primers
Literature & Tutorials:
This record last updated: 01-04-2007