PrimerX -- Automated design of mutagenic primers for site-directed mutagenesis
What you can do:
Design PCR primers for site-directed mutagenesis using DNA or protein sequences.
Highlights:
- PrimerX is a web-based program written to automate the design of mutagenic PCR primers for site-directed mutagenesis.
- Based on your input, PrimerX compares a template DNA sequence with a DNA or protein sequence that already incorporates the desired mutation.
- It then generates forward primer sequences by computing for all possible oligonucleotide sequences of appropriate length that encode this mutation and follow your specified constraints.
- Finally, PrimerX generates corresponding reverse primer sequences, and computes for other necessary information such as melting temperature and GC content for each primer pair.
- One option (recommended for generating specific SNPs and indels) is for you to enter a mutation in your template DNA sequence, so that the desired base pair insertions, deletions, or substitutions are incorporated.
- The other is to enter a mutation within the protein sequence encoded by your template DNA, in which case PrimerX generates mutagenic primers based on all possible DNA sequences that can encode the desired mutation, taking into account codon degeneracy. This is recommended for changing a specific amino acid residue into another.
Keywords:
- mutagenic PCR primers design tool
- site-directed mutagenesis primer design too
- SNP PCR primer design tool
- PCR primers
- degenerated PCR primers
Literature & Tutorials:
This record last updated: 03-28-2007