ZiFiT -- Zinc Finger Targeter
What you can do:
An engineered zinc finger/target site design tool.
Highlights:
- Zinc Finger Targeter (ZiFiT) is a simple and intuitive web-based tool that facilitates the design of zinc finger proteins (ZFPs) that can bind to specific DNA sequences.
- The current version of ZiFiT is based on a widely employed method of ZFP design, the 'modular assembly' approach, in which pre-existing individual zinc fingers are linked together to recognize desired target DNA sequences.
- Several research groups have described experimentally characterized zinc finger modules that bind many of the 64 possible DNA triplets.
- ZiFiT leverages the combined capabilities of three of the largest and best characterized module archives by enabling users to select fingers from any of these sets.
- ZiFiT searches a query DNA sequence for target sites for which a ZFP can be designed using modules available in one or more of the three archives.
- In addition, ZiFiT output facilitates identification of specific zinc finger modules that are publicly available from the Zinc Finger Consortium.
Keywords:
- Binding Sites
- DNA-Binding Proteins
- Protein Conformation
- Protein Engineering
- Secondary Protein Structure
- Tertiary Protein Structure
- Zinc Fingers
Literature & Tutorials:
This record last updated: 06-02-2008