AsiDesigner -- exon-based siRNA design server considering alternative splicing
What you can do:
An siRNA design program based on exon-based siRNA design algorithm considering alternative splicing.
Highlights:
- AsiDesigner is a web-based siRNA design software system, which provides siRNA design capability to account for alternative splicing for mRNA level gene silencing.
- It provides numerous novel functions including the designing of common siRNAs for the silencing of more than two mRNAs simultaneously, a scoring scheme to evaluate the performance of designed siRNAs by adopting currently known key design factors, a stepwise off-target searching with BLAST and FASTA algorithms and checking the folding secondary structure energy of siRNAs.
- To do this, we developed a novel algorithm to evaluate the common target region, where siRNAs can be designed to knockdown a specific mRNA isoform or more than two mRNA isoforms from a target gene simultaneously.
- The developed algorithm and the AsiDesigner were tested and validated as very effective throughout widely performed gene silencing experiments.
Keywords:
- siRNA design
- alternative splicing
- gene silencing
- mRNA silencing
- siRNA
- siRNA performance
Literature & Tutorials:
This record last updated: 07-21-2008