OHM -- OligoHeatMap
What you can do:
An online tool to estimate and display hybridizations of oligonucleotides onto DNA sequences.
Highlights:
- The efficiency of molecular methods involving DNA/DNA hybridizations depends on the accurate prediction of the melting temperature (T(m)) of the duplex.
- Many softwares are available for T(m) calculations, but difficulties arise when one wishes to check if a given oligomer (PCR primer or probe) hybridizes well or not on more than a single sequence.
- Moreover, the presence of mismatches within the duplex is not sufficient to estimate specificity as it does not always significantly decrease the T(m).
- OHM (OligoHeatMap) is an online tool able to provide estimates of T(m) for a set of oligomers and a set of aligned sequences, not only as text files of complete results but also in a graphical way: T(m) values are translated into colors and displayed as a heat map image, either stand alone or to be used by softwares such as TreeDyn to be included in a phylogenetic tree.
Keywords:
- oligomers
- DNA hybridization
- oligonucleotides
- DNA melting temperature
- T(m)
Literature & Tutorials:
This record last updated: 04-17-2014