siDRM -- an effective and generally applicable online siRNA design tool

What you can do:
Select effective siRNAs.
Highlights:
  • Small interfering RNAs have become an indispensable tool for the investigation of gene functions.
  • Most existing siRNA design tools were trained on datasets assembled from confined origins, incompatible with the diverse siRNA laboratory practice to which these tools will ultimately be applied.
  • We have performed an updated analysis using the disjunctive rule merging (DRM) approach on a large and diverse dataset compiled from siRecords, and implemented the resulting rule sets in siDRM, a new online siRNA design tool.
  • siDRM also implements a few high sensitivity rule sets and fast rule sets, links to siRecords, and uses several filters to check unwanted detrimental effects, including innate immune responses, cell toxic effects and off-target activities in selecting siRNAs.
  • A performance comparison using an independent dataset indicated that siDRM outperforms 19 existing siRNA design tools in identifying effective siRNAs.
Keywords:
  • siRNA
  • small interfering RNA
  • siRNA design
This record last updated: 09-03-2008
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