MFEprimer -- multiple factor evaluation of the specificity of PCR primers
What you can do:
Evaluate the specificity of PCR primers.
Highlights:
- MFEprimer evaluates the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3'-end of the primer, melting temperature, GC content and number of binding sites between the primer and DNA templates.
- MFEprimer can help the user to select more suitable primers before running either standard or multiplex PCR reactions.
- The cDNA and genomic DNA databases of 10 widely used species, as well as user custom databases, were used as DNA templates for analyzing primers specificity.
- A Primer3Plus server is maintained with a modified Primer3Manager for one-stop primer design and specificity checking.
Keywords:
- PCR
- polymerase chain reaction
- primer design
- primer analysis
- primer sequence
- primer specificity
- primer
Literature & Tutorials:
This record last updated: 08-16-2021