MFEprimer -- multiple factor evaluation of the specificity of PCR primers

What you can do:
Evaluate the specificity of PCR primers.
Highlights:
  • MFEprimer evaluates the specificity of PCR primers based on multiple factors, including sequence similarity, stability at the 3'-end of the primer, melting temperature, GC content and number of binding sites between the primer and DNA templates.
  • MFEprimer can help the user to select more suitable primers before running either standard or multiplex PCR reactions.
  • The cDNA and genomic DNA databases of 10 widely used species, as well as user custom databases, were used as DNA templates for analyzing primers specificity.
  • A Primer3Plus server is maintained with a modified Primer3Manager for one-stop primer design and specificity checking.
Keywords:
  • PCR
  • polymerase chain reaction
  • primer design
  • primer analysis
  • primer sequence
  • primer specificity
  • primer
This record last updated: 08-16-2021
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