GUIDANCE -- Guide-tree based alignment confidence server

URL:

http://guidance.tau.ac.il/

What you can do:

Evaluate the accuracy of multiple sequence alignment.

Highlights:

GUIDANCE is a web-server for the identification of unreliable alignment regions.
The web-server accepts as input a set of unaligned sequences.
The server aligns the sequences and provides a simple graphic visualization of the confidence score of each column, residue and sequence of an alignment, using a color-coding scheme.
The method is generic and the user is allowed to choose the alignment algorithm (ClustalW, MAFFT and PRANK are supported) as well as any type of molecular sequences (nucleotide, protein or codon sequences).
The server implements two different algorithms for evaluating confidence scores: (i) the heads-or-tails (HoT) method, which measures alignment uncertainty due to co-optimal solutions; (ii) the GUIDANCE method, which measures the robustness of the alignment to guide-tree uncertainty.
The server projects the confidence scores onto the MSA and points to columns and sequences that are unreliably aligned.
These can be automatically removed in preparation for downstream analyses.

Keywords:

Literature & Tutorials:

This record last updated: 07-15-2010