Map large expressed sequence tag (EST) and cDNA datasets to genomic DNA.
Highlights:
The web server hosts large collections of EST sequences (e.g. 4.1 million mouse ESTs of 1.87 Gb as of 2003) in precomputed indexed data structures for efficient sequence comparison.
Users can upload a genomic DNA sequence of interest and rapidly compare this to the complete collection of ESTs on the server.
It delivers a mapping of the ESTs on the genomic DNA. The e2g web interface provides a graphical overview of the mapping. Alignments of the mapped EST regions with parts of the genomic sequence are visualized. Zooming functions allow the user to interactively explore the results.
Mapped sequences can be downloaded for further analysis.