Primer Design Assistant (PDA) -- a web-based primer design tool
What you can do:
Design and evaluate PCR primers.
Highlights:
- Primer Design Assistant (PDA) is a web interface primer design service combined with thermodynamic theory to evaluate the fitness of primers.
- Advanced options on 5' GC content, 3' GC content, dimer check and hairpin check are available.
- The option of covered region constrains the PCR product to cover a user-defined segment.
- PDA accepts single sequence query or multiple ones in FASTA format.
- It produces optimal and homogeneous primer pairs that meet the need in experimental design with large-scaled PCR amplifications.
- Considering the system loading, the size of a submitted sequence is limited to 10 kb and the total sequence number in a query is limited to 20.
Keywords:
- PCR primers
- PCR primer design tool
- Polymerase Chain Reaction primers design tool
- PCR primers evaluation tool
Literature & Tutorials:
This record last updated: 06-16-2005